LABORATORY OF METHODS IN BIOCHEMISTRY AND MICROBIOLOGY (SVB0072)
Docente:
Anno:
3° anno
Corso di studi:
Scienze Biologiche D.M. 270
MATERIALE DIDATTICO
AA 2022/2023
Esercitazioni
Lezioni
- Biochemistry 1: Introduction and buffers
- Biochemistry 2: cell lysis, extraction, precipitation
- Biochemistry 3. Centrifugation, precipitation and dialysis
- Biochemistry 4: protein quantitation
- Biochemistry 5: chromatography intro
- Biochemistry 6: affinity chromatography and TLC
- Biochemistry 7: chromatography IEXC and gel permeation
- Biochemistry 8: Electrophoresis techniques
- Biochemistry 9: spectroscopy for enzyme activity and protein structure
AA 2021/2022
Lezioni
- Biochemistry 1: Introduction and buffers
- Biochemistry 2: cell lysis, extraction, precipitation
- Biochemistry 3. Centrifugation, precipitation and dialysis
- Biochemistry 4: protein quantitation
- Biochemistry 5: chromatography intro
- Biochemistry 6: affinity chromatography and TLC
- Biochemistry 7: chromatography IEXC and gel permeation
- Biochemistry 8: Electrophoresis techniques
- Biochemistry 9: spectroscopy for enzyme activity and protein structure
AA 2020/2021
Lezioni
- Biochemistry 10: HPLC background
- Biochemistry 2: cell lysis, extraction, precipitation
- Biochemistry 3. Centrifugation, precipitation and dialysis
- Biochemistry 4: protein quantitation
- Biochemistry 5: chromatography intro
- Biochemistry 6: affinity chromatography and TLC
- Biochemistry 7: chromatography IEXC and gel permeation
- Biochemistry 8: Electrophoresis techniques
- Biochemistry 9: spectroscopy for enzyme activity and protein structure
AA 2019/2020
Esercitazioni
- Appl_Micro_Laboratory_Example_of_elaborate
- Appl_Micro_Laboratory_protocol_01_media_prep
- Appl_Micro_Laboratory_protocol_02_total_viable_count_estimation
- Appl_Micro_Laboratory_protocol_03_antibiogram
- Appl_Micro_Laboratory_protocol_04_API
- Appl_Micro_Laboratory_protocol_05_bacterial_transformation
- Appl_Micro_Laboratory_protocol_06_PCR_RFLP_and_ARDRA
- BIOCHEMISTRY MODULE: Final lab reports evaluations/ Valutazioni finali reports di laboratorio
Lezioni
- Applied microbiology-L00-introduction
- Applied microbiology-L01: isolation and microscopy
- Applied microbiology-L02-biochemical_and_molecular_methods
- Applied microbiology-L03-MOs_and_health
- Applied microbiology-L04-industrial_microbiology
- Applied microbiology-L05_environmental_microbiology
- Applied microbiology-L06_vaccines
- Applied microbiology-L07_NGS
- Biochemistry 10: spectroscopy for enzyme activity and protein structure
- Biochemistry 11: bioelectrochemistry, electrodes and techniques
- Biochemistry 2: cell lysis, extraction, precipitation
- Biochemistry 3: centrifugation, concentration, dialysis
- Biochemistry 4: protein quantitation
- Biochemistry 5: chromatography intro
- Biochemistry 6: chromatography IEXC and gel permeation
- Biochemistry 7: affinity chromatography and TLC
- Biochemistry 8: HPLC
- Biochemistry 9: electrophoresis techniques
AA 2018/2019
Articoli
Dispense
Esercitazioni
- Calcoli HPLC esercitazione
- Codici_RData
- Esercitazione_3_dati
- Lab groups - Microbiology
- Protocols for lab practice - microbiology
- R code to install packages
- SDS PAGE picture Shift A (groups A,B,C,D,E)
- SDS PAGE picture Shot B (groups F,G,H,I,J,K)
- Template and guide for Lab report
- Turni laboratorio metodologie Biochimiche
Lezioni
- 10 spectroscopy
- 1_Introduction and parameters for in vitro exp design
- 2_Cell lysis, extraction, precipitation
- 3_ centrifugation, concentration, dialysis
- 4. Spectrophotometry and protein assay
- 5_Chromatography intro
- 6_Chromatography IEXC and gelperm
- 7_Affinity chromatography and TLC
- 8_HPLC
- 9. electrophoresis
AA 2017/2018
Articoli
Esercitazioni
Lezioni
- 10 spectroscopy
- 4. Spectrophotometry and protein assay
- 9. electrophoresis
- Modulo biochimica: 1. Introduzione e soluzioni tampone
- Modulo biochimica: 10 elettrochimica
- Modulo biochimica: 2. lisi e estrazione
- Modulo biochimica: 3. centrifugazione e precipitazione
- Modulo biochimica: 4. cromatografia di affinità
- Modulo biochimica: 6. cromatografia intro, IEXC, gel filtrazione
- Modulo biochimica: 8. HPLC
AA 2016/2017
Lezioni
- 10 spectroscopy
- 4. Spectrophotometry and protein assay
- 9. electrophoresis
- Modulo biochimica: 1. Introduzione e soluzioni tampone
- Modulo biochimica: 2. lisi e estrazione
- Modulo biochimica: 3. centrifugazione e precipitazione
- Modulo biochimica: 4. cromatografia di affinità
- Modulo biochimica: 6. cromatografia intro, IEXC, gel filtrazione
- Modulo biochimica: 8. HPLC
AA 2015/2016
Lezioni
- 4. Spectrophotometry and protein assay
- 9. electrophoresis
- Modulo biochimica: 1. Introduzione e soluzioni tampone
- Modulo biochimica: 2. lisi e estrazione
- Modulo biochimica: 3. centrifugazione e precipitazione
- Modulo biochimica: 4. cromatografia di affinità
- Modulo biochimica: 6. cromatografia intro, IEXC, gel filtrazione